Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin.
Identifieur interne : 000140 ( Main/Exploration ); précédent : 000139; suivant : 000141Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin.
Auteurs : Marcos C. Knirsch [Brésil] ; Filippo Dell'Anno [Italie] ; Dennis Chicoma [Brésil] ; Marco Antonio Stephano [Brésil] ; Nádia A. Bou-Chacra [Brésil] ; Domenico Palombo [Italie] ; Attilio Converti [Italie] ; Bronislaw Polakiewicz [Brésil]Source :
- Bioprocess and biosystems engineering ; 2015.
Abstract
Many complications are associated to the therapeutic use of blood, among which are not only transfusion adverse events but also other issues such as lack of donors and high costs for collecting, testing, preserving, and distributing blood packages. Therefore, a clinically viable "blood substitute" is considered the holy grail of traumatology and may greatly benefit medicine. One of the most successful approaches to date is conjugating hemoglobin with polyethylene glycol (PEG). This conjugation aims mainly at overcoming free cell hemoglobin toxicity, which makes its use as oxygen carrier in pure form unfeasible. To improve PEG-hemoglobin conjugates feasibility, we propose applying dual functional PEG cross-linking hemoglobin molecules encapsulated by a protein carrier. The new oxygen carrier showed mean values of the hydrodynamic diameter, dispersity, and zeta potential of 1370 nm, 0.029 and -36 mV, respectively, evidencing the successful synthesis of PEG bis(N-succinimidyl succinate) and polyhemoglobin as well as the structuring of protein carrier.
DOI: 10.1007/s00449-015-1463-y
PubMed: 26314399
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000060
- to stream PubMed, to step Curation: 000060
- to stream PubMed, to step Checkpoint: 000065
- to stream Ncbi, to step Merge: 000D93
- to stream Ncbi, to step Curation: 000D93
- to stream Ncbi, to step Checkpoint: 000D93
- to stream Main, to step Merge: 000137
- to stream Main, to step Curation: 000140
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin.</title><author><name sortKey="Knirsch, Marcos C" sort="Knirsch, Marcos C" uniqKey="Knirsch M" first="Marcos C" last="Knirsch">Marcos C. Knirsch</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Dell Anno, Filippo" sort="Dell Anno, Filippo" uniqKey="Dell Anno F" first="Filippo" last="Dell'Anno">Filippo Dell'Anno</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa</wicri:regionArea><wicri:noRegion>Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Chicoma, Dennis" sort="Chicoma, Dennis" uniqKey="Chicoma D" first="Dennis" last="Chicoma">Dennis Chicoma</name><affiliation wicri:level="4"><nlm:affiliation>Chemical Engineering Department, Polytechnic School, University of São Paulo, Av. Prof. Lineu Prestes 580 B18, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Chemical Engineering Department, Polytechnic School, University of São Paulo, Av. Prof. Lineu Prestes 580 B18, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Stephano, Marco Antonio" sort="Stephano, Marco Antonio" uniqKey="Stephano M" first="Marco Antonio" last="Stephano">Marco Antonio Stephano</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Bou Chacra, Nadia A" sort="Bou Chacra, Nadia A" uniqKey="Bou Chacra N" first="Nádia A" last="Bou-Chacra">Nádia A. Bou-Chacra</name><affiliation wicri:level="4"><nlm:affiliation>Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580 B15, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580 B15, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Palombo, Domenico" sort="Palombo, Domenico" uniqKey="Palombo D" first="Domenico" last="Palombo">Domenico Palombo</name><affiliation wicri:level="1"><nlm:affiliation>Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical Vascular Biology, DISC, Genoa University and IRCCS San Martino Hospital, Largo Rosanna Benzi, 16132, Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical Vascular Biology, DISC, Genoa University and IRCCS San Martino Hospital, Largo Rosanna Benzi, 16132, Genoa</wicri:regionArea><wicri:noRegion>Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Converti, Attilio" sort="Converti, Attilio" uniqKey="Converti A" first="Attilio" last="Converti">Attilio Converti</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy. converti@unige.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa</wicri:regionArea><wicri:noRegion>Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Polakiewicz, Bronislaw" sort="Polakiewicz, Bronislaw" uniqKey="Polakiewicz B" first="Bronislaw" last="Polakiewicz">Bronislaw Polakiewicz</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26314399</idno><idno type="pmid">26314399</idno><idno type="doi">10.1007/s00449-015-1463-y</idno><idno type="wicri:Area/PubMed/Corpus">000060</idno><idno type="wicri:Area/PubMed/Curation">000060</idno><idno type="wicri:Area/PubMed/Checkpoint">000065</idno><idno type="wicri:Area/Ncbi/Merge">000D93</idno><idno type="wicri:Area/Ncbi/Curation">000D93</idno><idno type="wicri:Area/Ncbi/Checkpoint">000D93</idno><idno type="wicri:Area/Main/Merge">000137</idno><idno type="wicri:Area/Main/Curation">000140</idno><idno type="wicri:Area/Main/Exploration">000140</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin.</title><author><name sortKey="Knirsch, Marcos C" sort="Knirsch, Marcos C" uniqKey="Knirsch M" first="Marcos C" last="Knirsch">Marcos C. Knirsch</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Dell Anno, Filippo" sort="Dell Anno, Filippo" uniqKey="Dell Anno F" first="Filippo" last="Dell'Anno">Filippo Dell'Anno</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa</wicri:regionArea><wicri:noRegion>Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Chicoma, Dennis" sort="Chicoma, Dennis" uniqKey="Chicoma D" first="Dennis" last="Chicoma">Dennis Chicoma</name><affiliation wicri:level="4"><nlm:affiliation>Chemical Engineering Department, Polytechnic School, University of São Paulo, Av. Prof. Lineu Prestes 580 B18, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Chemical Engineering Department, Polytechnic School, University of São Paulo, Av. Prof. Lineu Prestes 580 B18, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Stephano, Marco Antonio" sort="Stephano, Marco Antonio" uniqKey="Stephano M" first="Marco Antonio" last="Stephano">Marco Antonio Stephano</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Bou Chacra, Nadia A" sort="Bou Chacra, Nadia A" uniqKey="Bou Chacra N" first="Nádia A" last="Bou-Chacra">Nádia A. Bou-Chacra</name><affiliation wicri:level="4"><nlm:affiliation>Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580 B15, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580 B15, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Palombo, Domenico" sort="Palombo, Domenico" uniqKey="Palombo D" first="Domenico" last="Palombo">Domenico Palombo</name><affiliation wicri:level="1"><nlm:affiliation>Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical Vascular Biology, DISC, Genoa University and IRCCS San Martino Hospital, Largo Rosanna Benzi, 16132, Genoa, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical Vascular Biology, DISC, Genoa University and IRCCS San Martino Hospital, Largo Rosanna Benzi, 16132, Genoa</wicri:regionArea><wicri:noRegion>Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Converti, Attilio" sort="Converti, Attilio" uniqKey="Converti A" first="Attilio" last="Converti">Attilio Converti</name><affiliation wicri:level="1"><nlm:affiliation>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy. converti@unige.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa</wicri:regionArea><wicri:noRegion>Genoa</wicri:noRegion></affiliation></author><author><name sortKey="Polakiewicz, Bronislaw" sort="Polakiewicz, Bronislaw" uniqKey="Polakiewicz B" first="Bronislaw" last="Polakiewicz">Bronislaw Polakiewicz</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author></analytic><series><title level="j">Bioprocess and biosystems engineering</title><idno type="e-ISSN">1615-7605</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Many complications are associated to the therapeutic use of blood, among which are not only transfusion adverse events but also other issues such as lack of donors and high costs for collecting, testing, preserving, and distributing blood packages. Therefore, a clinically viable "blood substitute" is considered the holy grail of traumatology and may greatly benefit medicine. One of the most successful approaches to date is conjugating hemoglobin with polyethylene glycol (PEG). This conjugation aims mainly at overcoming free cell hemoglobin toxicity, which makes its use as oxygen carrier in pure form unfeasible. To improve PEG-hemoglobin conjugates feasibility, we propose applying dual functional PEG cross-linking hemoglobin molecules encapsulated by a protein carrier. The new oxygen carrier showed mean values of the hydrodynamic diameter, dispersity, and zeta potential of 1370 nm, 0.029 and -36 mV, respectively, evidencing the successful synthesis of PEG bis(N-succinimidyl succinate) and polyhemoglobin as well as the structuring of protein carrier.</div></front></TEI><affiliations><list><country><li>Brésil</li><li>Italie</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Knirsch, Marcos C" sort="Knirsch, Marcos C" uniqKey="Knirsch M" first="Marcos C" last="Knirsch">Marcos C. Knirsch</name></region><name sortKey="Bou Chacra, Nadia A" sort="Bou Chacra, Nadia A" uniqKey="Bou Chacra N" first="Nádia A" last="Bou-Chacra">Nádia A. Bou-Chacra</name><name sortKey="Chicoma, Dennis" sort="Chicoma, Dennis" uniqKey="Chicoma D" first="Dennis" last="Chicoma">Dennis Chicoma</name><name sortKey="Polakiewicz, Bronislaw" sort="Polakiewicz, Bronislaw" uniqKey="Polakiewicz B" first="Bronislaw" last="Polakiewicz">Bronislaw Polakiewicz</name><name sortKey="Stephano, Marco Antonio" sort="Stephano, Marco Antonio" uniqKey="Stephano M" first="Marco Antonio" last="Stephano">Marco Antonio Stephano</name></country><country name="Italie"><noRegion><name sortKey="Dell Anno, Filippo" sort="Dell Anno, Filippo" uniqKey="Dell Anno F" first="Filippo" last="Dell'Anno">Filippo Dell'Anno</name></noRegion><name sortKey="Converti, Attilio" sort="Converti, Attilio" uniqKey="Converti A" first="Attilio" last="Converti">Attilio Converti</name><name sortKey="Palombo, Domenico" sort="Palombo, Domenico" uniqKey="Palombo D" first="Domenico" last="Palombo">Domenico Palombo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000140 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000140 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= OperaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26314399
|texte= Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26314399" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OperaV1
| This area was generated with Dilib version V0.6.21. |  |